Differential Contributions of mSWI/SNF Chromatin Remodeler Sub-Families to Myoblast Differentiation

Mammalian SWI/SNF (mSWI/SNF) complexes are ATP-dependent chromatin remodeling enzymes that critical for normal cellular functions. mSWI/SNF classified into three sub-families based on the presence of specific subunit proteins. The Brm- or Brg1-associated factor (BAF), ncBAF (non-canonical BAF), and polybromo-associated BAF (PBAF). biological roles different enzyme poorly described. We knocked down expression genes encoding unique proteins each sub-family, Baf250A, Brd9, Baf180, which mark BAF, ncBAF, PBAF sub-families, respectively, examined requirement in myoblast differentiation. found Baf250A complex were required to drive lineage-specific gene expression. KD Brd9 delayed However, while Baf250A-dependent profile included myogenic genes, Brd9-dependent did not, suggesting indirectly contributed Baf180 was dispensable results distinguish between during